Allgemeine Pharmakologie und Toxikologie -
Prof. Dr. Josef Pfeilschifter

 

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Expertise

Krebs:

  • Grundlegende Kenntnis Virus-assoziierter Tumoren (EBV, HPV) als Modellsysteme für die Entwicklung tumorspezifischer Therapieansätze
  • Identifizierung von Tumorsuppressor-Regionen und genetischen Läsionen mittels Monochromosomen-transfer, Mikrosatellitenpolymorphismen-Analyse und SSCP-Analyse
  • Verbesserung von Strategien zur Krebsbehandlung: Screening für Synergismen bei der Kombination individueller Therapieformen (prodrug converting-Systeme zur Erhöhung der Strahlenempfindlichkeit: Verwendung spezifischer Virostatika)
  • Design und Evaluierung von tumorspezifischen Gentherapievektoren (basierend auf Adeno-, Retro- oder Herpesviren, präsent als artefizielle Minichromosomen; Design von Expressionskassetten,die von viralen Transkriptionsfaktoren gesteuert werden oder Promotoren aberrant exprimierter Gene enthalten; Exploration des therapeutischen Potentials von Suizidgenen, pro-apoptotischen und Tumorsuppressor-genen)
  • Ánalgetika (NSAIDs) für die Krebstherapie, Entwicklung lokaler tumorspezifischer Applikationsverfahren für diese Substanzen zur Reduktion gefährlicher Nebenwirkungen
  • Tumorigenitätstests an immundefizienten Mausstämmen; Rekultivierung von Tumormaterial in vitro

Entzündung:

  • Weit reichendes Verständnis der molekularen Mechanismen von Entzündungen der Niere und der Haut
  • Erforschung der Entzündungsprozesse während der Regeneration von Geweben: Wundheilung in der Haut als Modellsystem; Einfluss von Stickstoffmonoxid, Diabetes Typ II, Leptin, pharmakologische Interventionsmöglichkeiten bei Heilungsprozessen; Biologie der Keratinozyten
  • Die extrazelluläre Matrix (ECM) als Target während der Entzündung: Regulation von ECM-abbauenden Enzymen (Matrix-Metalloproteinasen (MMPs), (endogene) Plasminogenaktivatoren (PAs); Untersuchung der RNA-Stabilität als posttranskriptioneller Mechanismus zur Regulation der Genexpression
  • Kulturen von menschlichem Vollblut und von aus Blut frisch isolierten peripheren mononukleären Zellen: ein etabliertes Modellsystem für die Evaluierung der Produktion pro- und anti-entzündlicher Zytokine in vitro
  • Menschliche Colonkarzinomzellen als Modell zur Erforschung von Mechanismen der pro-entzündlichen und pro-apoptotischen Aktivierung
  • Untersuchung von Lokalisation und Funktion von Phospholipase A2-Subtypen in gesunder Haut und in der Haut von Patienten mit allergischen oder Autommunerkrankungen (Phospholipase A2-Aktivitäts-Assays (zytosolische und sekretorische Subformen) in vitro und an intakten Zellen)

Signatransduktion:

  • Analyse von Lipidmediatoren (radioaktiv, GC-MS, EIA)
  • Lipidanalyse mittels TLC
  • Detektion von PKC Isoenzym-vermittelten zellulären Funktionen (Mesangiumzellen als Modellsystem; in vitro PKC Isoenzym-Aktivitätsassays)
  • Quantifizierung von Tetrahydrobiopterin
  • Quantifizierung von Sphingolipiden (u. a. in humanem Gewebe) und Identifizierung von Signalkaskaden, an denen sie beteiligt sind
  • Identifizierung von Stickstoffmonoxid-regulierten Genen: Analyse differentieller Genexpression auf RNA (RAP-PCR)- und Proteinlevel

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Methoden und Geräte

  • Klonierung, DNA-Sequenzierung, Transformation / Transfektion, Infektion unter Verwendung viraler Vektoren
  • Zellsorting mittels FACS
  • Virusproduktion und –aufkonzentrierung durch Ultrazentrifugation
  • Immunhistochemie

- Immunzytochemie
- Immunfluoreszenz an Gewebeschnitten (Haut, Niere)

  • Konfokale Lasermikroskopie
  • Quantitative Realtime PCR
  • Luziferase-Assays (Promotoranalyse, Analyse von mRNA stabilisierenden Sequenzelementen durch Einklonieren von 3´ UTRs in Reportergene)
  • Electrophoretic mobility shift assays: DNA-/ RNA-Protein-Interaktionen
  • Chromatin-Immunopräzipitierung
  • Site-directed mutagenesis (funktionelle Analyse definierter cis-regulatorischer Promotorelemente)
  • In vitro RNA-Degradierungsassay (mRNA Stabilitätsmessungen)
  • Gelatin-Zymografie (semiquantitative Messungen von MMP-2- / MMP-9-Aktivitäten in Zellkultur-überständen)
  • Differenzielle Genexpressionsanalyse mittels 2D-Gelelectrophorese und differential display (RAP-PCR)
  • In-vitro-Modelle:
    - Glomeruläre Mesangiumzellen, MCF-7-Mammakarzinomzellen
    - Kulturen von menschlichem Vollblut und von aus Blut frisch isolierten peripheren mononukleären Zellen
    - Menschliche Burkittlymphomlinien als Modell für Virus-assoziierte Tumoren
    - Primäre Mauskeratinozyten: Isolierung und Kultivierung aus neonatalen Mäusen; mononukleäre Zellen
    - Menschliche Kolonkarzinomzellen: Mechanismen pro-inflammatorischer und pro apoptotischer Aktivierung
    - Isolierung humaner PBMCs
    - Kultivierung primärer humaner Keratinozyten; Zellinien etabliert aus Keratinozytentumoren
  • Tiermodelle:
    - C57BL/6, BALB/c, db/db, ob/ob, transgene Mäuse
    - Nackt- und SCID-Mäuse

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Mitarbeiter

  • PD Dr. K.-F. Beck/Prof. Dr. J. Pfeilschifter: (Genexpression in Abhängigkeit von Stickstoffmonoxid)
  • Dr. U Christen: Immunpharmakologie von Autoimmunerkrankungen
  • PD Dr. W. Eberhardt: Extrazelluläre Matrix und Entzündung
  • PD Dr. S. Frank: Entzündung, Wundheilung, Diabetes
  • Dr. P. Gutwein: Regulierung des ADAM-vermittelten Ektodomänen-Shedding von transmembran Chemokinen und Adhäsionsmolekülen in entzündlichen Nierenerkrankungen
  • PD Dr. A. Huwiler/ Prof. Dr. J. Pfeilschifter: Untersuchungen zu Streß-aktivierten Proteinkinasen und MAP-Kinasen im Verlauf von Entzündungsprozessen/ Rolle von Sphingolipiden in der glomerulären Pathophysiologie
  • Prof Dr. D. Meyer zu Heringdorf: Lipid-Signalwege
  • PD Dr. H. Mühl: Analyse der immunregulatorischen Cytokine IFNg, IL-18 und IL-22 im humanen System (Signaltransduktion, Entzündung)
  • Prof. Dr. H. Radecke: Chemokine und chronische Entzündung
  • Prof. Dr. L. Schäfer: Rolle extrazellulärer Matrix-Komponenten in der renalen Pathophysiologie

 

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Publikationen

2008

  • Lichte K, Rossi R, Danneberg K, ter Braak M, Kürschner U, Jakobs KH, Kleuser B, Meyer zu Heringdorf D: Lysophospholipid receptor-mediated calcium signaling in human keratinocytes. J Invest Dermatol 2008; 1:1-12.
  • Fink K, Zellweger R, Weber J, Manjarrez-Orduno N, Holdener M, Senn BM, Hemgartner H, Zinkernagel RM, Macpherson AJ: Long-term maternal imprinting of the specific B cell repertoire by maternal antibodies. Eur J Immunol 2008; 38:90-101.
  • Daniel C, Sartory NA, Zahn N, Radeke HH, Stein J: Immunomodulatory treatment of TNBS colitis with calcitriol is associated with a change of Th1/Th17 to Th2 and regulatory T- cell profile. J Pharmacol Exp Ther 2008; 324:23-33.
  • Cevikbas F, Schaefer L, Uhlig P, RobenekH, Theilmeier G, Echtermeyer F, Bruckner P: Unilateral nephrectomy leads to upregulation of syndecan-2 and TGF-beta mediated glomerulosclerosis in syndecan-4 deficient mice. Matrix Biol 2008; 27:42-52.
  • Johann AM, Weigert A, Eberhardt W, Kuhn A-M, Barra V, von Knethen A, Pfeilschifter J, Brüne B: Apoptotic cell derived S1P promotes HuR-dependent COX-2 mRNA stabilization and protein expression. J Immunol 2008; 180:1239-1248.
  • Conen A, Weisser M, Orasch JC, Battagay M, Trampuz A, Frei R, Christen S, Heim D, Gratwohl A, Flückiger U: Candida krusei - a serious complication in patients with hematological malignancies: successful treatment with caspofungin. Transpl Infect Dis 2008; 10:66-70.
  • Hoegl S, Boost KA, Flondor M, Scheiermann P, Muhl H, Pfeilschifter J, Zwissler B, Hofstetter C: Short-term exposure to high-pressure ventilation leads to pulmonary biotrauma and systemic inflammation in the rat. Int J Mol Med 2008; 21:513-519.
  • Rubant SA, Ludwig RJ. Diehl S, Hardt K, Kaufmann R. Pfeilschifter JM, Boehncke WH: Dimethylfumarate reduces leukocyte rolling in vivo through modulation of adhesion molecule expression. J Invest Dermatol 2008; 128:326-331.
  • Westermann D, Mersmann J, Melchior A, Petrik C, Freudenberger T, Lettau O, Schaefer L, Lüllmann-Rauch R, Jacoby C, Schrader J, Brand-Herrmann SM, Young MF, Levkau B, Babba H, Unger T, Zacharowski K, Tschöpe C, Fischer JW: Biglycan is required for adaptive remodelling after myocardial infarction. Circulation 2008; 117:1269-1276.
  • Schwalm S, Graf F, Römer I, Bubnova S, Pfeilschifter J, Huwiler A: Sphingosine kinase-1 is a hypoxia-regulated gene that regulates migration of human endothelial cells. Biochem Biophys Res Commun 2008; 368:1020-1025.
  • Doller A, Akool ES, Huwiler A, Müller R, Radeke HH, Pfeilschifter J, Eberhardt W: Posttranslational modification of the AU-rich element binding protein HuR by PKCdelta elicits angiotensin II-induced stabilization and nuclear export of COX-2 mRNA. Mol Cell Biol 2008; 28:2608-2625.
  • Poleganov MA, Bachmann M, Pfeilschifter J, Mühl H: Genome-wide analysis displays marked induction of EBI3/IL-27B in IL-18-activated AML-derived KG1 cells: Critical role of two kappaB binding sites in the human EBI3 promotor. Mol Immunol 2008; 45:2869-2880.
  • Koch A, König B, Stangl GI, Eder K: PPAR alpha mediates transcriptional upregulation of novel organic cation transporters-2 and -3 and enzymes involved in hepatic carnitine synthesis. Exp Biol Med (Maywood) 2008; 233:356-365.
  • König B, Koch A, Giggel K, Dordschbal B, Eder K, Stangl GI: Monocarboxylate transporter (MCT)-1 is up-regulated by PPARalpha. Biochim Biophys Acta 2008; 1780:899-904.
  • Lastres-Becker I, Brodesser S, Lütjohann D, Azizov M, Buchmann J, Hintermann E, Sandhoff K, Schürmann A, Nowock J, Auburger G: Insulin receptor and lipid metabolism pathology in ataxin-2 knock-out mice. Hum Mol Genet 2008; 15:1465-1481.
  • Janot L, Secher T, Torres D, Maillet I, Pfeilschifter J, Quesniaux VFJ, Landmann, R, Ryffel B, Erard F: CD14 with Toll-like receptor 2 contributes to recognition and control of Listeria monocytogenes infection. J Infect Dis 2008; 198:115-124.
  • Huwiler A Pfeilschifter J: New player on the center stage: sphingosine 1-phosphate and its receptors as drug targets. Biochem Pharmacol 2008; 75:1893-1900.
  • Schramme A, Abdel-Bakky MS, Kämpfer-Kolb N, Pfeilschifter J, Gutwein P: The role of CXCL16 and its processing metalloproteinases ADAM10 and ADAM17 in the proliferation and migration of human mesangial cells. Biochem Biophys Res Commun 2008; 370:311-316.
  • Schramme A, Abdel-Bakky MS, Gutwein P, Obermüller N, Baer PC, Hauser IA, Ludwig A, Gauer S, Schäfer L, Sobkowiak E, Altevogt P, Koziolek M, Kiss E, Gröne HJ, Tikkanen R, Goren I, Radeke HH, Pfeilschifter J: Characterization of CXCL16 and ADAM10 in the normal and transplanted kidney. Kidney Int 2008; 74:328-338.
  • Oh ST, Schramme A, Stark A, Tilgen W, Gutwein P, Reichrath J: Overexpression of ADAM10 and ADAM12 in lesional psoriatic skin. Br J Dermatol 2008; 158:1371-1373.
  • Schiefelbein D, Goren I, Fißlthaler B, Schmidt H, Geisslinger G, Pfeilschifter J, Frank S: Biphasic regulation of HMG CoA reductase expression and activity during wound healing and its functional role in the control of keratinocyte angiogenic and proliferative responses. J Biol Chem 2008; 283:15479-15490.
  • Sadik CD, Hunfeld KP, Bachmann M, Kraiczy P, Eberhardt W, Brade V, Pfeilschifter J, Mühl H: Systematic analysis highlights the key role of TLR2/NFkappaB/MAP kinase signaling for IL-8 induction by macrophage-like THP-1 cells under influence of B.burgdorferi lysates. Int J Biochem Cell Biol 2008; 40:2508-2521.
  • Schaefer L, Iozzo RV: Biological functions of the Small leucine-rich proteoglycans: From genetics to signal transduction. J Biol Chem 2008; 283:21305-21309.
  • Holdener M, Hintermann E, Bayer M, Rhode A, Rodrigo E, Hintereder G, Johnson EF, Gonzalez FJ, Pfeilschifter J, Manns MP, von Herrath MG, Christen U: Breaking tolerance to the natural human liver autoantigen Cytochrome P450 2D6 by virus infection. J Exp Med 2008; 205:1409-1422.
  • Akool ES, Doller A, Babelova A, Tsalastra W, Moreth K, Schaefer L, Pfeilschifter J, Eberhardt W: Molecular mechanisms of TGFb-receptor-triggered signalingn cascades rapidly induced by the calcineurin inhibitors cyclosphorine and FK506. J Immunol 2008; 181:2831-2845.
  • Goren I, Müller E, Pfeilschifter, Frank S: Thr308 determines Akt1 nuclear localization in insulin-stimulated keratinocytes. BBRC 2008; 372:103-107.

2007

  • Keller C, Rivera GiP, Tölle M, van der Giet M, Chun J, Radeke HH, Schäfer-Korting M, Kleuser B: Immunmodulator FTY720 induces myofibroblast differentiation via the lysophospholipid receptor S1P3 and Smad3 signalling. Am J Pathol 2007; 170:281-290.
  • Waibler Z, Kalinke U, Will J, Herrero San Juan M, Pfeilschifter JM, Radeke HH: TLR-ligand stimulated interleukin-23 subunit expression and assembly is regulated differentially in murine plasmacytoid and myeloid dendritic cells. Mol Immunol 2007; 44:1483-1489.
  • Moreth K, Riester D, Hildmann C, Hempel R, Wegener D, Schober A, Schwienhorst A: An active site tyrosine residue is essential for amidohydrolase but not for esterase activity of a class 2 histone deacetylase-like bacterial enzyme. Biochem J 2007; 401:659-665.
  • Bachmann M, Dragoi C, Poleganov MA, Pfeilschifter J, Mühl H: Interleukin-18 directly activates T-bet expression and function via p38-MAP kinase and NFkappaB in AML-derived Pre-dendritic KG-1 cells. Mol Cancer Ther 2007; 6:723-731.
  • Daniel C, Sartory NA, Zahn N, Geisslinger G, Radeke HH, Stein J: FTY720 ameriolates Th1-mediated colitis in mice by directly affecting the functional activity od CD4+CD25+ regulatory T-cells. J Immunol 2007; 178:2458-2468.
  • Ramos-Lopez E, Brück P, Jansen T, Pfeilschifter JM, Radeke HH, Badenhoop K: CYP2R1-, CYP27B1- and CYP24-mRNA expression in German type 1 diabetes patients. J Steroid Biochem Mol Biol 2007; 103:807-810.
  • Buchhaupt M, Pfeifer C, Entian KD: Analysis of 2`-O-methylated nucleosides and pseudouridines in ribosomal RNAs using DNAzymes. Anal Biochem 2007; 361:102-108.
  • Schaefer L, Tsalastra W, Babelova A, Baliova M, Minneruo J, Sorokin L, Gröne HJ, Reinhardt DP, Pfeilschifter J, Iozzo RV, Schaefer RM: Decorin-mediated regulation of fibrillin-1 in the kidney involves IGF-1 receptor and mTOR. Am J Pathol 2007; 170:301-315.
  • Bedke J, Kiss E, Schaefer L, Behnes CL, Bonrouhi M, Gretz N, Horuk R, Diedrichs-Moehring M, Wildner G, Nelson PJ, Gröne HJ: Beneficial effects of CCR1 blockade on the progression of chronic renal allograft-damage. Am J Transplant 2007; 7:527-537.
  • Klawitter S, Hofmann LP, Pfeilschifter J, Huwiler A: Extracellular nucleotides induce migration of renal mesangial cells by upregulating sphingosine kinase-1 expression and activity. Br J Pharmacol 2007; 150:225-257.
  • Stoeck A, Gast D, Sanderson MP, Issa Y, Gutwein P, Altevogt P: L1-CAM in a membrane-bound or soluble form augments protection from apoptosis in ovarian carcinoma cells. Gynecol Oncol 2007; 104:461-469.
  • Doller A, Akool ES, Müller R, Gutwein P. Kurowski C, Pfeilschifter J, Eberhardt W: Molecular Mechanisms of cyclosporin A inhibition of the cytokine-induced matrix metalloproteinase-9 (MMP-9) in glomerular mesangial cells. J Am Soc Nephrol 2007; 18:581-592.
  • Zhang C, Wenger T, Mattern J, Ilea S, Frey C, Gutwein P, Altevogt P., Bodenmuller W, Gassler N, Schnabel PA, Dienemann H, Marme A, Hohenfellner M, Haferkamp A, Pfitzenmaier J, Grone HJ, Kolb A, Buchler P, Buchler M, Friess H, Rittgen W, Edler L, Debatin KM, Krammer PH, Rutz HP, Herr I: Clinical and Mechanistic Aspects of Glucocorticoid-Induced chemotherapy resitance in the majority of solid tumors. Cancer Biol Ther 2007; 6:278-287.
  • Boost KA, Hoegl S, Hofstetter C, Flondor M, Stegewerth K, Platacis I, Pfeilschifter J, Mühl H, Zwissler B: Targeting caspase-1 by inhalation-therapy: effects of Ac-YVAD-CHO on Il-1b, IL-18 and downstream pro-inflammatory parameters as detected in rat ecdotoxaemia. Int Care Med 2007; 33:863-871.
  • Eberhardt W, Doller A, Akool ES, Pfeilschifter J: Modulation of mRNA stability as a novel therapeutic approach. Pharmacology & Therapeutics 2007; 114:56-73.
  • Doller A, Huwiler A, Müller R, Radeke HH, Pfeilschifter J, Eberhardt W: PKCalpha-dependend phosphorylation of the mRNA stabilizing factor HuR: implications for posttranscriptional regulation of cyclooxygenase-2. Mol Biol Cell 2007; 18:2137-2148.
  • Ziesché E, Bachmann M, Kleinert H, Pfeilschifter J, Mühl H: The IL-22/STAT3 pathway potentiates expression of iNOS in human colon carcinoma cells. J Biol Chem 2007; 282:16006-16015.
  • Döll F, Pfeilschifter J, Huwiler A: Prolactin upregulates sphingosine kinase-1 expression and activity in the human breast cancer cell line MCF7 and triggers enhanced proliferation and migration. Endocrine-Related Cancer 2007; 14:325-335.
  • Daniel C, Sartory NA, Zahn N, Schmidt R, Geisslinger G, Radeke HH, Stein J: FTY720 ameliorates oxazolone-coliltis in mice by directly affecting T helper 2 functions. Mol Immunol 2007; 44:3305-3316.
  • Eberhardt W, Pfeilschifter J: Nitric oxide and vascular remodeling: Spotlight on the kidney. Kidney Int Suppl 2007; 106:S9-S16.
  • Schacke W, Beck KF, Pfeilschifter J, Hattenbach LO: Expression of ubiquitin carboxy-terminal hydrolase-11 in photocoagulated human retinal pigment epithelial cells. Curr Eye Res 2007; 32:367-372.
  • Goren I, Müller E, Schiefelbein D, Christen U, Pfeilschifter J, Mühl H, Frank S: Systemic anti-TNFalpha treatment restores diabetes-impaired skin repair in ob/ob mice by inactivation of macrophages. J Invest Derm 2007; 127:2259-2267.
  • Luchtefeld M, Bandlow N, Tietge UJ, Grote K, Pfeilschifter J, Kaszkin M, Beck S, Drexler H, Schieffer B: Angiotensin II type 1-receptor antagonism prevents type IIA secretory phospholipase A(2)-dependent lipid peroxidation. Atherosclerosis 2007; 194:62-70.
  • Gauer S, Sichler O, Obermüller N, Holzmann Y, Kiss E, Sobkowiak E, Pfeilschifter J, Geiger H, Mühl H, Hauser IA: IL-18 is expressed in the intercalated cell of human kidney. Kidney Int 2007; 72:1081-1087.
  • Behrens F, Himsel A, Rehart S, Stanczyk J, Beutel B, Zimmermann SY, Köhl U, Moeller B, Gay S, Kaltwasser JP, Pfeilschifter JM, Radeke HH: Imbalance in distribution of functional autologous regulatory T cells in rheumatoid arthritis. Annals of the Rheumatic Diseases 2007; 66:1151-1156.
  • Buchhaupt M, Kotter P, Entian KD: Mutations in the nucleolar proteins Tma23 and Nop6 suppress the malfunction of the Nep1 protein. FEMS Yeats Res 2007; 7:771-781.
  • Schaefer L, Schaefer RM: A primer on iron therapy. Nephrol Dial Transplant, 22:2429-2431.
  • Ulrich S, Huwiler A, Loitsch S, Schmidt H, Stein JM: De novo ceramide biosysnthesis is associated with resveratrol-induced inhibition or ornithine decarboxylase activity. Biochem Pharmacol 2007; 74:281-289.
  • Xin C, Ren S, Eberhardt W, Pfeilschifter J, Huwiler A: Sphingosylphosphorylcholine acts in an anti-inflammatory manner in renal mesangial cells by reducing interleukin-1beta - induced prostaglandin E2 formation. J Lipd Res 2007; 48:1985-1996.
  • Dwir O, Grabovski V, Pasvolsky R, Manevich E, Shamri R, Gutwein P, Feigelson SW, Altevogt P, Alon R: Membranal cholestreol is not required for L-selectin adhesiveness in primary lymphocytes but controls a chemokine-induced destabilization of L-selectin rolling adhesions. J Immunol 2007; 179:1030-1038.
  • Nold M, Nold-Petry C, Fischer D, Richter B, Blaheta R, Pfeilschifter J, Mühl H, Schranz D, Veldman A: Activated protein C downregulates p38 mitogen-activated protein kinase and improves clinical parameters in an in vivo model of septic shock. Thrombosis and Haemostatsis 2007; 98:1118-1126.
  • Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P: CD24 is a marker of exosomes secreted into urine and amniotic fluid. Kidney Int 2007; 72:1095-1102.
  • Hintermann E, Christen U: Viral infection - a cure for type 1 diabetes? Cur Med Chem 2007; 14: 2048-2052.
  • Christen U, Hintermann E: Dual role of CXCL10 as conductor of cellular traficking during typ 1 diabetes. Int J Biomed Sci 2007; 3:86-92.
  • Christen U, Holdener M, Hintermann E: Animal models for autoimmune hepatitis. Autoimmune Rev 2007; 6: 306-311.
  • Christen U: Chemokines as drug targets intype 1 diabetes. Endocr Metab Immun Disord Drug Targets (Curr Drug Targets) 2007; 7:7-12.
  • Martinic MM, Juedes AE, Bresson D, Homann D, Skak K, Huber C, Ling E, Ejrnares M, Wolfe T, Togher L, Christen U, von Herrath MG: Minimal impact of a de-novo expressed B-cell autoantigen on spontaneous diabetes development in NOD mice. Diabetes 2007; 56:1059-1068.
  • Schneider D, Liaw L, Daniel C, Athanasopoulus AN, Herrmann M, Preissner KT, Nawroth PP, Chavakis T: Inhibition of breasr cancer cell adhesion and bone metastasis by the extracellular adherence protein of Staphylococcus aureus. Biochem Biophys Res Com 2007; 357:282-288.
  • Daniel C, Schroeder O, Zahn N, Steinhilber D, Stein J: The TGFbeta-Smad 3-signalling pathway is involved in butyrate-mediated Vitamin D receptor expression. J Cell Biochem 2007; 102:1420-1431.
  • Xin C, Ren S, Eberhardt W, Pfeilschifter J, Huwiler A: FTY720 suppresses Interleukin -1beta induced secretory phospholipase A2 expression in renal mesangial cells by a transriptional mechanism. Br J Pharmacol 2007; 150:943-950.
  • König B, Koch A, Spielmann J, Hilgenfeld C, Stangl GI, Eder K: Activation of PPARalpha lowers synthesis and concentration of cholesterol by reduction of nuclear SREBP-2. Biochem Pharmacol 2007; 73:574-585.
  • Koch A, König B, Spielmann J, Leitner A, Stangl, GI Eder K: Thermally oxidized oil increases the expression of insulin-induced genes and inhibits activation of sterol regulatory elememt-binding protein-2 in rat liver. J Nutr 2007; 137:2018-2023.
  • Koch A, König B, Luci S, Stangl, GI, Eder K: Dietary oxidzed fat up regulates the expression of organic cation transporters in liver and small intestine and alters carnitine concentrations in liver, muscle and plasma of rats. Br J Nutr 2007; 98:882-889.
  • Hofstetter C, Boost KA, Flondor M, Basagan-Mogol E, Betz C, Homann M, Mühl H, Pfeilschifter J, Zwissler B: Anti-inflammatory effects of sevoflurane and mild hypothermia in endotoxemic rats. Acta Anaesthesiol Scand 2007; 51:893-899.
  • Boost KA, Flondor M, Hofstetter C, Platacis I, Stegewerth K, Hoegl S, Nquyen T, Mühl H, Zwissler B:. The beta-adrenoceptor antagonist propranolol counteracts anti-inflammatory effects of isoflurane in rat endotoxemia. Acta Anaesthesiol Scand 2007; 51:900-908.
  • Hofstetter C, Boost KA, Hoegl S, Flondor M. Scheller B, Mühl H, Pfeilschifter J, Zwissler B: Norepinephrine and vasopressin counteract anti-inflammatory effects of isoflurane in endotoxemic rats. Int J Mol Med 2007; 20:597-604.
  • Alemany R, van Koppen CJ, Danneberg K, Ter Braak M, and Meyer Zu Heringdorf D: Regulation and functional roles of sphingosine kinases. Naunyn Schmiedebergs Arch Pharmacol 2007; 374:413-428.
  • Meyer Zu Heringdorf D, Jakobs KH: Renal mesangial cells: moving on sphingosine kinase-1. Br J Pharmacol 2007; 150:255-257.
  • Meyer Zu Heringdorf D, Jakobs KH: Lysophospholipid receptors: signalling, pharmacology and regulation by lysophospholipid metabolism. Biochim Biophys Acta 2007; 1768:923-940.
  • Mewes KR, Raus M, Bernd A, Zöller NN, Sättler A, Graf R: Elastin expression in a newly developed full thickness skin equivalent. Skin Pharmacol Physiol 2007; 20:85-95.
  • Poleganov MA, Pfeilschifter J, Mühl H: Expanding extracellular zinc beyond levels reflecting the albumin-bound plasma zinc pool potentiates the capability of IL-1beta, IL-18, and IL-12 to act as IFNgamma-inducing factors on PBMC. J Interferon Cytokine Res 2007; 27:997-1002.
  • Huwiler A, Zamgemeister-Wittke U: Targeting the conversion of ceramide to sphingosine 1-phosphate as a novel strategy for cancer therapy. Crit Rev Oncol Hemat 2007; 63:150-159.

2006

  • Bachmann M, Kosan C, Xing PX, Montenarh M, Hoffmann I, Möröy T: The oncogenic serine/threonine kinase Pim-1 directly phosphorylates and activates the G2/M specific phosphatase Cdc25C. Int J Biochem Cell Biol 2006; 38:430-443.
  • Zhang C, Marme A, Wenger T, Gutwein P, Edler L, Rittgen W, Debatin KM, Altevogt P, Mattern J, Herr I: Glucocorticoid-mediated inhibition of chemotherapy in ovarian carcinomas. Int J Oncol 2006; 28:551-558.
  • Bachmann M, Horn K, Poleganov MA, Paulukat J, Nold M, Pfeilschifter J, Mühl H: Interleukin-18 secretion and Th1-like cytokine responses in human peripheral blood mononuclear cells under the influence of the toll-like receptor-5 ligand flagellin. Cellular Microbiology 2006; 8,289-300.
  • Plesková M, Beck KF, Behrens MH, Huwiler A, Fichtlscherer B, Wingerter O,Brandes RP, Mülsch A, Pfeilschifter J: Nitric oxide down-regulates the expression of the catalytic NADPH oxidase subunit Nox1 in rat renal mesangial cells. FASEB J 2006; 20,139-141.
  • Xin C, Ren S, Eberhardt W, Pfeilschifter J, Huwiler A: The immunomodulator FTY720 and its phosphorylated derivative activate the Smad signaling cascade and upregulate connective tissue growth factor (CTGF) and collagen type IV expression in renal mesangial cells. Br J Pharmacol 2006; 147:164-74.
  • Goren I, Linke A, Müller E, Pfeilschifter J, Frank, S: The Suppressor of Cytokine Signaling (SOCS)-3 is upregulated in impaired skin repair: Implications for keratinocyte proliferation. J Invest Dermatol 2006;126:477-85.
  • Goren I, Müller E, Pfeilschifter J, Frank, S: Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of TNFalpha. Am J Pathol 2006; 168:765-77.
  • Goren I, Kämpfer H, Müller E, Schiefelbein D, Pfeilschifter J, Frank, S: Oncostatin M expression is functionally connected to neutrophils in the early inflammatory phase of skin repair: implications for normal and diabetis-impaired wounds. J Invest Dermatol 2006; 126:628-37.
  • Ludwig RJ, Werner RJ, Winker W, Boehncke WH, Wolter M, Kaufmann R: Chronic venous insufficiency - a potential trigger for localized scleroderma J Eur Acad Dermatol Venereol 2006; 20:96-99.
  • Stoeck A, Keller S, Riedle S, Sanderson MP, Runz S, Lenaour F, Gutwein P, Ludwig A, Rubinstein E, Altevogt P: A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44. Biochem J 2006; 393:609-618.
  • Stirnweiss J, Valkova C, Ziesche E, Drube S, Liebmann C: Muscarinic M2 receptors mediate transactivation of EGF receptor through Fyn kinase and without matrix metalloproteases. Cell Signal 2006; 18:1338-1349.
  • Stoeck A, Schlich S, Issa J, Gschwend V, Wenger T, Herr I, Marme A, Bourbie S, Altevogt P, and Gutwein P: L1 on ovarian carcinoma cells is a binding partner for Neuropilin-1 on mesothelial cells. Cancer Letters 2006; 239:212-26.
  • Mühl H, Pfeilschifter J: Controlling the cytokine storm by insulin: Glycogen Synthase Kinase-3 as a target in systemic inflammation. Crit Care Med 2006; 34:1567-1569.
  • Huwiler A, Döll F, Ren S, Klawitter S, Greening A, Römer I, Bubnova S, Reinsberg L, Pfeilschifter J: Histamine increases sphingosine kinase-1 expression and activity in the human arterial endothelial cell line EA.hy 926 in a PKC-alpha dependend mechanism. Biochim. Biophys. Acta - Molecular and Cell Biology of Lipids 2006; 1761:367-376.
  • Boost KA, Hofstetter C, Flondor M, Betz C, Homann M, Pfeilschifter J, Mühl H, Zwissler B: Desflurane differentially affects the release of proinflammatory cytokines in plasma and bronchoalveolar fluid of endotoxemic rats. Int J Mol Med 2006; 17:1139-1144,
  • Ludwig RJ, Alban S, Bistrian R, Boehncke WH, Henschler R, Kaufmann R, Gille J: The ability of different forms of heparins to suppress P-selectin function in vitro correlates to their inhibitory capacity on blood-borne metastasis in vivo. Thromb Haemost 2006; 95:535-40.
  • Rubant S, Ludwig RJ, Pfeffer J, Schulze-Johann P, Kaufmann R, Pfeilschifter JM, Boehncke WH, Radeke HH: Eukaryotic expression of the broad-spectrum chemokine receptor antagonist vMIP-II and its effects on T-cell function in vitro and in vivo. Exp Dermatol 2006; 15:634-42.
  • Fritzsche J , Alban S, Ludwig RJ, Rubant S, Boehncke WH, Schumacher G, Bendas G: The influence of various structural parameters of semisynthetic sulfated polysaccharides on the P-selectin inhibition capacity. Biochemical Pharmacology 2006; 72:474-85,
  • Wienrich BG, Oostingh GJ, Ludwig RJ, Enders S, Harms G, Tauber R, Krahn T, Kramer B, Boehncke WH, Schön MP: reply to "Efomycine M: an inhibitor of selections ?". Nat Med 2006; 12:873-874,
  • Beck S, Beck G, Ostendorf T, Floege J, Lambeau G, Nevalainen T, Radeke HH, Gurrieri S, Haas U, Thorwart B, Pfeilschifter J, Kaszkin M: Upregulation of group IB secreted phospholipase A2 and its M-type receptor in rat anti-Thy-1 glomerulonephritis. Kidney Int 2006; 70:1251-1260.
  • Huwiler A, Pfeilschifter J: Altering the Sphingosine-1-phosphate/Ceramide Balance: A Promising Approach for Tumor Therapy? Curr Pharmaceut Design 2006; 12:4625-4635.
  • Dequiedt F, Martin M, von Blume J, Vertommen D, Lecomte E, Mari N, Heinen MF, Bachmann M, Twizere JC, Huang C, Rider MH, Piwnica-Worms H, Seufferlein T, Kettmann R: A new role for hPar-1 kinases, EMK and C-TAK1 in regulating localization and activity of class IIa histone deacetylases. Mol Cell Biol 2006; 26:7086-7102,
  • Hartner A, Schaefer L, Porst M, Cordasic N, Gabriel N, Klanke B, Reinhardt DP, Hilgers KF: Role of fibrillin-1 for hypertensive and diabetic glomerular disease. Am J Physiol Renal Physiol 2006; 290:F1329-F1336.
  • Kurylowicz A, Ramos-Lopez E, Bednarczuk T, Badenhoop K: Vitamin D-binding protein (DBP) gene polymorphism is associated with Graves`disease and the vitamin D status in a Polish population study. Exp Clin Endocrinol Diabetes 2006; 114:329-325.
  • Ramos-Lopez E, Ghebru S, van Autreve J, Aminkeng F, Herwig J, Seifried E, Seidl C, van der Auwera B, Badenhoop K: Neither an intronic CA repeat within the CD48 gene nor the HERV-K18 polymorphisms are associated with type 1 diabetes. Tissue Antigens 2006; 68:147-152.
  • Lieb B, Dimitrova K, Kang HS, Braun S, Gebauer W, Martin A, Hanelt B, Saenz SA, Adema CM, Markl J: Red blood with blue-blood ancestry: intriguing structure of a snail hemoglobin. Proc Natl Acad Sci USA 2006; 103:12011-12016
  • Huwiler A, Pfeilschifter J: Hypoxia and lipid signaling. Biol. Chem 2006; 387:1321-1328.
  • Ludwig RJ, Alban S, Boehncke WH: Structural requirements of modified glycosaminoglycans to exert polyvalent anti-inflammatory activities. Mini-Rev Med Chem 2006; 6:1009-1023.
  • Rüster B, Gottig S, Ludwig RJ, Bistrian R, Müller S, Seifried E, Gille J, Henschler R: Mesenchymal stem cells (MSC) display coordinated rolling and adhesion behaviour on endothelial cells. Blood 2006; 108:3938-3944.
  • Ramos-Lopez E, Jansen T, Ivaskevicius V, Kahles H, Klepzig C, Oldenburg J, Badenhoop K: Protection from type 1 diabetes by vitamin D receptor haplotypes.Ann N Y Acad Sci 2006; 1079:327-334.
  • Daniel C, Radeke HH, Sartory NA, Zuegel U, Steinmeyer A, Stein J: The new low calcemic vitamin D analogue 22-ene-25-oxa-vitamin D ameliorates Th1-mediated colitis in mice. J Pharmacol Experimental Therapeutics 2006; 319:622-631.
  • Ludwig RJ, Schindewolf M, Utikal J, Lindhoff-Last E, Boencke WH: Management of cutanenous heparin hypersensitivity reactions. Thromb Haemost 2006; 96(5): 611-617.
  • Buchhaupt M, Meyer B, Kotter P, Entian KD: Genetic evidence for 18S rRNA binding and an Rpr19p assembly function of yeast nucleolar protein Nep1p. Mol Genet Genomics 2006; 276:273-284.
  • Hankeln T, Klawitter S, Kramer M, Burmester T: Molecular characterization of hemoglobin from the honeybee Apis mellifera. J Insect Physiol 2006; 52:701-710.
  • Burmester T, Storf J, Hasenjager A, Klawitter S, Hankeln T: The hemoglobin genes of Drosophila. FEBS J 2006; 273:468-480.
  • Fujinami RS, von Herrath MG, Christen U, Whitton JL: Molecular mimicry, bystander activation, or viral persistence: Infections and autoimmune disease. Clin Microbiol Rev 2006; 19:80-94.
  • Ejrnaes M, von Herrath MG, Christen U: Cure of chronic viral infection and virus-induced type-1 diabetes by neutralizing antibodies. Clin Dev Immunol 2006; 13:337-347.
  • Barral AM, Thomas HE, Ling EM, Darwiche R, Rodrigo E, Christen U, Ejrnaes M, Wolfe T, Kay TW, von Herrath MG: SOCS-1 protects from virally-induced CD8 T-cell mediated type 1 diabetes. J Autoimmun 2006; 27:166-173.
  • von Allmen N, Christen S, Forster U, Gottstein B, Welle M, Muller N: Acute trchinellosis increases susceptibilityto Giardia lamblia infection in the mouse model. Parasitology 2006; 133:139-149.
  • Fink, K, Lang KS, Manjarrez-Orduno N, Junt T, Senn BM, Holdener M, Akira S, Zinkernagel RM, Hengartner H: Early type I interferon-mediated signals on B cells specifically enhance antiviral humoral responses. Eur J Immunol 2006; 36:2094-2105.
  • Brück P, Vilches CN, Ramos-Lopez E, Barboza QO, Ancer RJ, Flores GJP: Expresion del Her2-Neu en el adenocarcinoma ductal de la glandula mamaria: correlacion con parametros istopatologicos y expresion de receptores estrogenicos en pacientes mexicanas. Ginecol Obstet Mex 2006; 74:516-522.
2005
  • Kautz K, Schwarz M, Radeke HH: Interference with MCP-1 gene expression by vector generated triple helix-forming RNA oligonucleotides. CMLS, Cell Mol Life Sci 2005; 62:001-015.
  • Djordjevic T, Belaiba RS, Bonello S, Pfeilschifter J, Hess J, Görlach A: Human Urotensin II Is a Novel Activator of NADPH Oxidase in Human Pulmonary Artery Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 2005; 25:519-525.
  • Vlachojannis GJ, Scholz-Pedretti K, Fierlbeck W, Geiger H, Pfeilschifter J, Kaszkin M: Enhanced expression of group IIA secreted phospholipase A2 by elevated glucose levels in cytokine-stimulated rat mesangial cells and in kidneys of diabetic rats. Clin Nephrol 2005; 63:356-367.
  • Gunia S, Behrens MH, Stosiek P: Mycobacterial spindle cell pseudotumour (MSP) of the nasal septum clinically mimicking Kaposi's sarcoma: A case report. Rhinology 2005; 43:70-71.
  • Bachmann M, Moroy T: The serine/threonine kinase Pim-1. Int J Biochem Cell Biol, 37:726-30.
  • Haas U, Podda M, Behne M, Gurrieri S, Alonso A, Fürstenberger G, Pfeilschifter J, Lambeau G, Gelb MH, Kaszkin M: Characterization and differentiation-dependent regulation of secreted phospholipases A2 in human keratinocytes and healthy and psoriatic human skin. J Invest Dermatol 2005; 124:204 -211.
  • Kämpfer H, Schmidt R, Geisslinger G, Pfeilschifter J, Frank S: Wound inflammation in diabetic ob/ob mice: functional coupling of prostaglandin biosynthesis to cyclooxygenase-1 activity in diabetes-impaired wound healing. Diabetes 2005; 54:1543-1551.
  • Hoth A, Johannisson R, Syed Ali S, Schulze J, Siegers C-P: Cytotoxicity of organotin compounds in different cultured cell lines. Toxicol. Industr. Health, 131-136, 2005
  • Beck KF, Güder G, Schaefer L, Pleskova M, Babelova A, Behrens MH, Mihalik D, Beck M, Schaefer RM, Pfeilschifter J: Nitric oxide upregulates induction of plateled-derived growth factor receptor a expression in rat renal mesangial cells and in anti-Thy-1 glomerulonephritis. J Am Soc Nephrol 2005; 16:1948-1957.
  • Hofstetter C, Flondor M, Boost KA, Koehler P, Bosmann M, Pfeilschifter J, Zwissler B, Mühl H: A brief exposure to Isoflurane (50 seconds) significantly impacts on plasma cytokine levels in endotoxemic rats. International Immunopharmacology 2005; 5:1519-1522.
  • Eberhardt W, Kilz T, Akool El-Sayed, Müller R, Pfeilschifter J: Dissociated Glucocorticoids equipotently inhibit cytokine- and cAMP-induced matrix degrading proteases in rat mesangial cells. Biochem Pharmacol 2005; 70:433-445.
  • Calderwood JW, Williams JM, Morgan MB, Nash GB, Savage CO: ANCA induces beta2 integrin and CXC chemokine-dependend neutrophil-endothelial cell interactions that mimic those of highly cytokine-activated endothelium. J Leukoc Biol 2005; 77:33-43.
  • Daniel C, Schlauch T, Zugel U, Steinmeyer A, Radeke HH, Steinhilber D, Stein J: 22-ene-25-oxa-vitamin D: a new vitamin D analogue with profound immunosuppressive capacities. Eur J Clin Invest 2005; 35:343-349.
  • Hauser IA, Spiegler S, Kiss E, Gauer S, Sichler O, Scheuermann EH, Ackermann H, Pfeilschifter JM, Geiger H, Grone HJ, Radeke HH: Prediction of acute renal allograft rejection by urinary monokine induced by IFNgamma (MIG). J Am Soc Nephrol 2005; 16:1849-1858.
  • Radeke HH, von Wenckstern H, Stoidtner K, Sauer B, Hammer S, Kleuser B: Overlapping signaling pathways of sphingosine 1-phosphate and TGF-beta in the murine langerhans cell line XS52. J Immunol 2005; 174:2778-2786.
  • Hofstetter C, Flondor M, Hoegl S, Mühl H, Zwissler B: Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat. Crit Care Med 2005; 33:2317-2322.
  • Petry C, Huwiler A, Eberhardt W, Kaszkin M, Pfeilschifter J: Hypoxia increases group IIA phospholipase A2 expression under inflammatory conditions in rat renal mesangial cells. J Am Soc Nephrol 2005; 16:2897-2905.
  • Christen U, von Herrath MG: Infections and autoimmunity--good or bad? J Immunol 2005; 174:7481-7486.
  • Ejrnaes M, Videbaek N, Christen U, Cooke A, Michelsen BK, von Herrath M: Different diabetogenic potential of autoaggressive CD8+ clones associated with IFN-gamma-inducible protein 10 (CXC chemokine ligand 10) production but not cytokine expression, cytolytic activity, or homing characteristics. J Immunol 2005; 174:2746-2755.
  • Hintermann E, Yang N, O'Sullivan D, Higgins JM, Quaranta V: Integrin alpha6beta4-erbB2 complex inhibits haptotaxis by up-regulating E-cadherin cell-cell junctions in keratinocytes. J Biol Chem 2005; 280:8004-8015.
  • Gutwein P, Stoeck A, Riedle S, Gast D, Runz S, Condon TP, Marme A, Phong MC, Linderkamp O, Skorokhod A, Altevogt P: Cleavage of L1 in exosomes and apoptotic membrane vesicles released from ovarian carcinoma cells. Clin Cancer Res 2005; 11:2492-2501.
  • Gast D, Riedle S, Riedle S, Schabath H, Schlich S, Schneider A, Issa Y, Stoeck A, Fogel M, Joumaa S, Wenger T, Herr I, Gutwein P, Altevogt P: L1 augments cell migration and tumor growth but not beta3 integrin expression in ovarian carcinomas. Int J Cancer 2005; 115:658-665.
  • Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Schulze Johann P, Pfeffer J, Radeke HH, Kaufmann R, Boehncke WH, Podda M: Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation. J Invest Dermatol 2005; 125:969-976.
  • Radeke HH, Ludwig RJ, Boehncke WH: Experimental approaches to lymphocyte migration in dermatology in vitro and in vivo. Experimental Dermatology 2005; 14:641-666.
  • Calderwood JW, Radeke HH: Chapter 4: In vitro techniques to investigate lymphocyte extravasation. In: “Lymphocyte homing to the skin” Boehncke WH (Ed.), CRC Press, Boca Raton, FL, USA, 2005
  • Rhode A, Pauza M, Barral AM, Rodrigo E, Oldstone MB, von Herrath MG, Christen U. Islet-specific expression of IP10 (CXCL10) targets lymphocytes to the pancreas and accelerate type 1 diabetes. J Immunol 2005; 175:3516-3524.
  • Hattenbach LO, Beck KF, Pfeilschifter J, Koch F, Ohrloff C, Schacke W. Pigment-Epithelium-Derived Factor is upregulated in photocoagulated human retinal pigment epithelial cells. Ophthalmic Res 2005; 37:341-346.
  • Akool ES, Doller A, Müller R, Gutwein P, Xin C, Huwiler A, Pfeilschifter J, Eberhardt W: Nitric oxide induces Timp-1 expression by activating the TGFß-Smad signaling pathway. J Biol Chem 2005; 280:39403-39416.
  • Haase H, Alvarez J, Petzhold D, Doller A, Behlke J, Erdmann J, Hetzer R, Regitz-Zagrosek V, Vassort G, Morano I: Ahnak is critical for cardiac Ca(v)1.2 calcium channel function and its [beta]-adrenergic regulation. FASEB J 2005; 19:1969-1977.
  • Christen U, von Herrath MG: Viruses as initiators, accelerators or terminators of autoimmunity?- A question of time and location of infection. Autoimmune Liver Disease (Falk Symposium 142), 2005
  • Pfeilschifter J, Beck KF: Chapter 15, pages: 327-340: Nitric oxide and gene expression. In " Nitric oxide cell signaling and gene expression", Cadenas E and Lamas S (Eds.), Taylor and Francis group, Boca Raton, Fl, USA, 2005
  • Ren S, Xin, C, Beck KF, Saleem MA, Mathieson P, Pavenstädt H, Pfeilschifter J, Huwiler A: PPARalpha activation upregulates nephrin expression in human embryonic kidney epithelial cells and podocytes by a dual mechanism. Biochim Biophys Res Commun 2005; 338:1818-24.
  • Döll F, Pfeilschifter J, Huwiler A: The epidermal growth factor stimulates sphingosine kinase-1 expression and activity in the human mammary carcinoma cell line MCF7. Biochim Biophys Acta 2005; 1738:72-81.
  • Wahab NA, Schaefer L, Weston BS, Yiannikouris O, Wright A, Babelova A, Schaefer R, Mason RM: Glomerular expression of thrombospondin-1., transforming growth factor beta and connective tissue growth factor at different stages of diabetic nephropathy and their interdependent roles in mesangial response to diabetic stimuli. Diabetologia 2005; 48:2650-2660.
  • Schaefer L, Babelova A, Kiss E, Hausser HJ, Baliova M, Krzyzankova M, Marsche G, Young MF, Mihalik D, Gotte M, Malle E, Schaefer RM, Grone HJ: The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages. J Clin Invest 2005; 115:2223-2233.
  • Heeg MH, Koziolek MJ, Vasko R, Schaefer L, Sharma K, Muller GA, Strutz F: The antifibrotic effects of relaxin in human renal fibroblasts are mediated in part by inhibition of the Smad2 pathway. Kidney Int 2005; 68:96-109.
  • Seidler DG, Schaefer L, Robenek H, Iozzo RV, Kresse H, Schönherr E: A physiologic three-dimensional cell culture system to investigate the role of decorin in matrix organisation and cell survival. Biochem Biophys Res Commun 2005; 15, 332:1162-1170.
  • Schönherr E, Sunderkötter C, Iozzo R, Schaefer L: Decorin, a novel player in the insulin-like growth factor system. J Biol Chem 2005; 280:15767-15772.

2004

  • Petry C, Fritz G, Pfeilschifter J, Huwiler A: Inhibition of Rho modulates cytokine- induced prostaglandin E2 formation in renal mesangial cells. Biochem Biophys Acta 2004; 1636:0108-118.
  • Chatterjee PK, Patel NSA, Cuzzocrea S, Brown PAJ, Stewart KN, Mota-Filipe H, Britti D, Eberhardt W, Pfeilschifter J, Thiemermann C: The cyclopentenone prostaglandin 15-deoxy-delta12, 14-prostaglandin J2 ameliorates ischemic acute renal failure. Cardiovasculare Research 2004; 61:630-643.
  • Kaszkin M, Beck KF, Eberhardt W, Pfeilschifter J: Unravelling Green Tea's Mechanisms of Action: More than Meets the Eye. Mol Pharmacol 2004; 65:15-17.
  • Hocher B, Schwarz A, Slowinski T, Bachmann S, Pfeilschifter J, Neumayer HH, Bauer C: In-vivo interaction of nitric oxide and endothelin. Journal of Hypertension 2004; 22:111-119.
  • Rodriguez I, Holloschi A, Kaszkin M, Cheng H, Kabsch K, Hafner M, Alonso A: Activation of phospholipase C-gamma1 in human keratinocytes by hyperosmolar shock without enzyme phosphorylation. Arch Dermatol Res 2004; 295:490-497.
  • Kaszkin M, Beck KF, Koch E, Erdelmeier C, Kusch S, Pfeilschifter J, Loew D: Downregulation of iNOS expression in rat mesangial cells by special extracts of Harpagophytum procumbens derives from harpagoside-dependent and independent effects. Phytomedicine 2004; 11:585-595.
  • Blume C, Sabuda-Widemann D, Pfeilschifter J, Plum J, Schror K, Grabensee B, Beck KF: Cerivastatin inhibits proliferation of interleukin-1beta-induced rat mesangial cells by enhanced formation of nitric oxide. Eur J Pharmacol 2004; 485:1-10.
  • Hellmuth M, Paulukat J, Ninic R, Pfeilschifter J, Mühl H: Nitric oxide differentially regulates pro- and anti-angiogenic markers in DLD-1 colon carcinoma cells. FEBS Lett 2004; 563:98-102.
  • Boissel JP, Ohly D, Bros M, Gödtel-Armbrust U, Förstermann U, Frank S: The neuronal nitric oxide synthase is upregulated in mouse skin repair and in responce to epidermal growth factor in human HaCaT keratinocytes. J Invest Dermatol 2004; 123:132-139.
  • Zampetaki A, Mitsialis SA, Pfeilschifter J, Kourembanas S: Hypoxia induces macrophage inflammatory protein-2 (MIP-2) gene expression in murine macrophages via NF-kB. FASEB J 2004; 18:1090-1092.
  • Gunia S, Behrens MH, Stosiek P: A rare course of Whipple`s disease with atypical cardiac manifestation. Acta Pathologica, Microbiologica, et Immunologica Scandinavica 2004; 112:222-224.
  • Fichtlescherer S, Kaszkin M, Breuer S, Dimmler S, Zeiher AM: Elevated secretory group II phospholipase A2 serum activity is assiciated with impaired endothelial vasodilator function in patients with coronary artery disease. Clin Sci (Lond) 2004; 106:511-517.
  • Xin C, Ren S, Kleuser B, Shabahang S, Eberhardt W, Radeke H, Schäfer-Korting M, Pfeilschifter J, Huwiler A: Sphingosine-1-phosphate cross-activates the Smad-signaling cascade and mimics TGFb-induced cell responses. J Biol Chem 2004; 279:35255-35262.
  • Huwiler A, Xin C, Brust AK, Briner VA, Pfeilschifter J: Differential binding of ceramide to MEKK1 in glomerular endothelial and mesangial cells. Biochim Biophys Acta 2004; 1636:159-168.
  • Huwiler A, Pfeilschifter J: Erythropoietin is more than just a promoter of erythropoiesis. J Am Soc Nephrol 2004; 15:2240-2241.
  • Mühl, H, Pfeilschifter J: Interleukin-18 bioactivity: a novel target for immunopharmacological anti-inflammatory intervention. Eur J Pharmacol 2004; 500:63-71.
  • Kahlina K, Goren I, Pfeilschifter J, Frank S: p68 dead box RNA helicase expression in keratinocytes: Regulation, nuclear localisation, and functional connection to proliferation and VEGF gene expression. J Biol Chem 2004; 279:44872-44882.
  • Xin C, Ren S, Pfeilschifter J, Huwiler A: Heterologous desensitivation of the sphingosine-1-phosphate receptors by purinoceptor activation in renal mesangial cells. Br J Pharmacol, 143:581-589.
  • Schäfer L, Ren S, Schäfer RM, Mihalik D, Babelova A, Huwiler A, Pfeilschifter J: Nephrin expression is increased in anti-Thy1.1-induced glomerulonephritis in rats. Biochem Biophys Res Commun, 5;324:247-254.
  • Mühl H, Paulukat J, Höfler S, Hellmuth M, Franzen R, Pfeilschifter J: The HIV protease inhibitor Ritonavir synergizes with butyrate for induction of apoptotic cell death and mediates expression of heme oxygenase-1 in DLD-1 colon carcinoma cells. Br J Pharmacol 2004; 143:890-898.
  • Sun M, Kong L, Wang X, Holmes C, Gao Q, Zhang G-R, Pfeilschifter J, Goldstein DS, Geller A: Coexpression of tyrosine hydroxylase, GTP cyclohydrolase I, aromatic amino acid decarboxylase, and vesicular monoamine transporter-2 from a helper virus-free HSV-1 vector supports high-level, long-term biochemical and behavioral correction of a rat model of Parkinson's disease. Human Gene Ther 2004; 15:1177-1196.
  • Bachmann M, Hennemann H, Xing PX, Hoffmann I, Moroy T: The oncogenic serine/threonine kinase Pim-1 phosphorylates and inhibits the activity Cdc25C-associated kinase 1 (C-TAK1): a novel role for Pim-1 at the G2/M cell cycle checkpoint. J Biol Chem 2004; 12;279:48319-48328.
  • Potapov EV, Ignatenko S, Nasseri BA, Loebe M, Harke C, Bettmann M, Doller A, Regitz-Zagrosek V, Hetzer R: Clinical significance of PlA polymorphism of platelet GP IIb/IIIa receptors during long-term VAD support. Ann Thorac Surg 2004; 77:869-874.
  • Fähling M, Perlewitz A, Doller A, Thiele BJ: Regulation of collagen prolyl4-hydroxylase and matrix metalloproteinases in fibrosarcoma cells by hypoxia. Comp Biochem Physiol C Toxicol Pharmacol 2004; 139:119-126.
  • Itoh K, Cheng L, Kamei Y, Fushiki S, Kamiguchi H, Gutwein P, Stoeck A, Arnold B, Altevogt P, Lemmon V: Brain development in mice lacking L1-L1 homophilic adhesion. J Cell Biol 165:145-154.
  • Thiele BJ, Doller A, Kähne T, Pregla R, Hetzer R, Regitz-Zagrosek V: RNA-Binding Proteins Heterogeneous Nuclear Ribonucleoprotein A1, E1, and K Are Involved in Post-Transcriptional Control of Collagen I and III Synthesis. Circ Res 2004; 95:1058-1066.
  • Kaszkin M, Schneider B, Loew D: Kasuistik: Ein hochdosierter Harpagophytum- Spezialextrakt als Phytoanalgetikum und -antiphlogistikum zur Remissionserhaltung des Morbus Crohn. Ärztezeitschrift fuer Naturheilverfahren 2004; 2:102-106.
    Kaszkin M, Pfeilschifter J, Loew D: Harpagophytum-Extrakte als sinnvolle Phytoanalgetika/-antiphlogistika? Schweizer Medizinische Zeitschrift für Phytotherapie 2004; 4:4-8.
  • Jiang X, Multhaupt H, Chan E, Schaefer L, Schaefer RM, Couchman JR: Essential contribution of tumor-derived perlecan to epidermal tumor growth and angiogenesis. J Histochem Cytochem 2004; 52:1575-1590.
  • Schönherr E, Sunderkötter C, Schaefer L, Thanos S, Grassel S, Oldberg A, Iozzo RV, Young MF, Kresse H: Decorin deficiency leads to impaired angiogenesis in injured mouse cornea. J Vasc Res 2004; 41:499-508.
  • Schaefer L, Mihalik D, Babelova A, Krzyzankova M, Grone HJ, Iozzo RV, Young MF, Seidler DG, Lin G, Reinhardt DP, Schaefer RM: Regulation of fibrillin-1 by biglycan and decorin is important for tissue preservation in the kidney during pressure-induced injury. Am J Pathol 2004; 65:383-396.
  • Yung S, Hausser H, Thomas G, Schaefer L, Kresse H, Davies M: Catabolism of newly synthesized decorin in vitro by human peritoneal mesothelial cells. Perit Dial Int 2004; 24:147-155.

2003

  • Akool E.S., Kleinert H., Hamada F.M.A., Abdelwahab M.H., Förstermann U., Pfeilschifter J, Eberhardt W: Nitric oxide increases the decay of MMP-9 mRNA by inhibiting the expression of mRNA stabilizing factor HuR. Mol Cell Biol 2003; 23:4901-4916.
  • Goren I, Kämpfer H, Podda M, Pfeilschifter J, Frank S: Leptin and wound inflammation in diabetic ob/ob mice: differential regulation of neutrophil and macrophage influx and a potential role for the scab as a sink for inflammatory cells and mediators. Diabetes 2003; 52:2821-2832.
  • Kämpfer H, Bräutigam L, Geisslinger G, Pfeilschifter J, Frank S: Cyclooxygenase-1-coupled prostaglandin biosynthesis constitutes an essential prerequisite for skin repair. J Invest Dermatol 2003; 120:880-890.
  • Nold M, Hauser IA, Höfler S, Goede A, Eberhardt W, Ditting T, Geiger H, Pfeilschifter J, Mühl H: IL-18BPa:Fc cooperates with immunosuppressive drugs in human whole blood. Biochem Pharmacol 2003; 66:501-506.
  • Mühl H, Höfler S, Pfeilschifter J: Inhibition of lipoplysaccharide/ATP-induced release of interleukin-18 by KN-62 and Glyburide. Eur J Pharmacol 2003; 482:325-328.
  • Schaefer L, Beck KF, Raslik I, Walpen S, Mihalik D, Micegova M, Macakova K, Schonherr E, Seidler DG, Varga G, Schaefer RM, Kresse H, Pfeilschifter J.: Biglycan, a nitric oxide-regulated gene, affects adhesion, growth and survival of mesangial cells. J Biol Chem 2003; 278:26227-26237.

2002

  • Feng WH, Westphal E, Mauser A, Raab-Traub N, Gulley ML, Busson P, Kenney SC: Use of adenovirus vectors expressing Epstein-Barr virus (EBV) immediate-early protein BZLF1 or BRLF1 to treat EBV-positive tumors. J Virol 2002; 76:10951-10959.
  • Eberhardt W, Schulze M, Engels C, Klasmeier E, Pfeilschifter J: Glucocorticoid-mediated suppression of cytokine-induced matrix metalloproteinase-9 expression in rat mesangial cells: involvement of NF-kB and Ets transcription factors. Mol Endocrinol 2002; 16:1752-1766.
  • Hellmuth M, Wetzler C, Nold M, Chang J-H, Frank S, Pfeilschifter J, Mühl H: Expression of Interleukin-8, Heme Oxygenase-1, and Vascular Endothelial Growth Factor in DLD-1 Colon Carcinoma Cells Exposed to Pyrrolidine Dithiocarbamate. Carcinogenesis 2002; 23:1273-1279.

2001

  • Walpen S, Beck KF, Schaefer L, Raslik I, Eberhardt W, Schaefer RM, Pfeilschifter J: Nitric oxide induces MIP-2 transcription in rat renal mesangial cells and in a rat model of glomerulonephritis. FASEB J 2001; 15:571-573.
  • Franzen R, Pautz A, Bräutigam L, Geisslinger G, Pfeilschifter J, Huwiler A: Interleukin 1beta induces chronic activation and de novo synthesis of neutral ceramidase in renal mesangial cells. J Biol Chem 2001; 276:35382-35389.

2000

  • Westphal EM, Ge J, Catchpole JR, Ford M, Kenney SC: The nitroreductase / CB1954 combination in Epstein-Barr virus-positive B-cell lines: induction of bystander killing in vitro and in vivo. Cancer Gene Ther 2000; 7:97-106.
  • Westphal EM, Blackstock W, Feng W, Israel B, Kenney SC: Activation of lytic Epstein-Barr virus infection by radiation and sodium butyrate in vitro and in vivo : a potential method for treating EBV-positive malignancies. Cancer Res 2000; 60:5781-5788.
  • Eberhardt W, Beck K-F, Huwiler A, Walpen S, Pfeilschifter J: Amplification of interleukin-1b-triggered MMP-9 expression in rat mesangial cells by superoxide is mediated by increased activity of NF-kB and AP-1 and involves activation of the MAPKs pathways. J Immunol 2000; 165:5788-5797.
  • Frank S, Stallmeyer B, Kämpfer H, Kolb, N, Pfeilschifter J: Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. J Clin Invest 2000; 106:501-509.



 
   

 

 

INSTITUTE UND MEDIZINISCHE ZENTREN

 
Chemie

Institut für Organische Chemie und Chemische Biologie

Prof. Dr. Joachim W. Engels

Prof. Dr. Michael Göbel

Prof. Dr. Gisbert Schneider

Pharmazie

Institut für Pharmazeutische Biologie

Prof. Dr. Theo Dingermann

Prof. Dr. Rolf Marschalek

Institut für Pharmazeutische Chemie

Prof. Dr. Michael Karas

Prof. Dr. Manfred Schubert-Zsilavecz

Prof. Dr. Holger Stark

Prof. Dr. Dieter Steinhilber

Institut für Pharmazeutische Technologie

Prof. Dr. Jennifer Dressman

Prof. Dr. Jörg Kreuter

Pharmakologisches Institut für Naturwissenschaftler

Prof. Dr. Jochen Klein

Prof. Dr. Walter E. Müller

Gustav-Embden-Zentrum für Biologische Chemie

Institut für
Biochemie I: Pathobiochemie

Prof. Dr. Bernhard Brüne

Zentrum der Hygiene

Medizinische Virologie

Prof. Dr. Hans Wilhelm Doerr

Dr. Senckenbergische Anatomie

Anatomie I: Klinische Neuroanatomie

Prof. Dr. Thomas Deller

Anatomie II: Experimentelle Neurobiologie

Prof. Dr. Horst-Werner Korf

Zentrum der Pharmakologie

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Allgemeine Pharmakologie und Toxikologie

Prof. Dr. Josef Pfeilschifter

Immunpharmakologie

Prof. Dr. Heinfried Radeke

Klinische Pharmakologie

Prof. Dr. Dr. Gerd Geißlinger

Zentrum der Physiologie

Physiologie I: Kardiovaskuläre Physiologie

Prof. Dr. Ralf Brandes

Institute for Vascular Signalling

Prof. Dr. Ingrid Fleming

Zentrum für Gesundheits-
wissenschaften

Allgemeinmedizin

Prof. Dr. Ferdinand Gerlach

Zentrum der Anästhesiologie und Wiederbelebung

Anästhesiologie, Intensivmedizin und Schmerztherapie

Prof. Dr. Oliver Habler

Prof. Dr. Paul Kessler

Zentrum der Chirurgie

Klinik für Allgemeinchirurgie

Prof. Dr. Wolf O. Bechstein

Dr. Christiane Gog

Dr. Christoph W. Strey

PD Dr. Christoph Wullstein

Mund-, Kiefer- und Plastische Gesichtschirurgie

Prof. Dr. Dr. Dr. Robert Sader

Zentrum der Dermatologie und Venerologie

Klinik für Dermatologie, Venerologie und Allergologie

Prof. Dr. Roland Kaufmann

Zentrum der Frauenheilkunde und Geburtshilfe

Klinik für Frauenheilkunde und Geburtshilfe

Prof. Dr. Manfred Kaufmann

Zentrum der Inneren Medizin

Med. Klinik I: Gastroenterologie und Hepatologie, Pneumologie und Allergologie, Endokrinologie und Diabetologie sowie Ernährungsmedizin

Prof. Dr. Christoph Sarrazin

PD Dr. Dr. Oliver Schröder

Prof. Dr. Gerlinde Teuber

Prof. Dr. Stefan Zeuzem

Med. Klinik II: Hämatologie / Onkologie, Rheumatologie, Infektiologie, Therapie der HIV - Erkrankung

Prof. Dr. Harald Burkhardt

Prof. Dr. Hubert Serve

Zentrum für Kinder- und Jugendmedizin

Klinik I: Stoffwechsel, Endokrinologie, Gastroenterologie, Ernährung, Pneumologie, Allergologie, Infektiologie, Neurologie und Neonatologie

Prof. Dr. Hansjosef Böhles

Prof. Dr. Stefan Zielen

Klinik für Pädiatrische Hämatologie, Onkologie und Hämostaseologie

Prof. Dr. Peter Bader

Prof. Dr. Thomas Klingebiel

Dr. Ulrike Köhl

Prof. Dr. Thomas Lehrnbecher

Prof. Dr. Dirk Schwabe

Zentrum der Neurologie und Neurochirurgie

Klinik für Neurologie

Prof. Georg Auburger

Prof. Rüdiger Hilker

Dr. H. Laufs

Dr. Matthias Lorenz

PD Dr. N. Müller

Prof. Dr. T. Neumann-Haefelin

Prof. Dr. Helmuth Steinmetz

Prof. Dr. Ulf Ziemann

Zentrum der Radiologie

Institut für Diagnostische und Interventionelle Radiologie

Prof. Dr. Thomas Vogl

Institut für Neuroradiologie

Prof. Dr. Friedhelm Zanella

Klinik für Nuklearmedizin

Prof. Dr. Frank Grünwald